验证数据展示
产品信息
88249-1-PBS targets Cleaved GSDME (Asp270), C-terminal in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), Indirect ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | deafness, autosomal dominant 5 |
| 别名 | Cleaved Gasdermin E, cleaved GSDME, cleaved GSDME (Asp270), DFNA5, DFNA5/GSDME |
| 计算分子量 | 496 aa, 55 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC019689 |
| 基因名称 | DFNA5 |
| Gene ID (NCBI) | 1687 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O60443 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
GSDME, also known as DFNA5 (deafness, autosomal dominant 5), is a member of the gasdermin family of pore-forming proteins. In its full-length form, GSDME is autoinhibited. Upon apoptotic stimulation, active caspase-3 specifically cleaves GSDME at a conserved aspartate residue, Asp270 (D270). This cleavage of Gasdermin E separates the N‑terminal pore‑forming domain from the C‑terminal repressor domain. The released N‑terminal fragment then inserts into the plasma membrane, forms large pores, and induces a lytic, inflammatory form of cell death known as pyroptosis. Therefore, Asp270 cleavage is a critical molecular switch that converts caspase‑3‑mediated apoptosis into pyroptosis, linking GSDME/DFNA5 to cancer therapy, chemotherapy‑induced toxicity, and hearing loss pathology.
