• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67784-1-Ig (same clone as 67784-1-PBS)

    WB analysis using 67784-1-Ig (same clone as 67784-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67784-1-Ig (citrate synthase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CS antibody included
  • IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CS antibody included
  • IF Staining of HepG2 using 67784-1-Ig (same clone as 67784-1-PBS)

    IF Staining of HepG2 using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    IF Staining of mouse breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67784-1-Ig (same clone as 67784-1-PBS)

    IF Staining of MCF-7 using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

citrate synthase Monoclonal antibody, PBS Only

citrate synthase Monoclonal Antibody for WB, IHC, IF/ICC, IF-Fro, Indirect ELISA
Cat No. 67784-1-PBS

产品说明书

CloneNo. 2F9G6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-Fro, Indirect ELISA

别名

CS, 2F9G6, CISY, Citrate, Citrate synthase, mitochondrial

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CS Ready-To-Use IHC Kit. CS primary antibody included.

产品信息

67784-1-PBS targets citrate synthase in WB, IHC, IF/ICC, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-Fro, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9255

Product name: Recombinant human CS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 132-466 aa of BC010106

Sequence: EQVSWLSKEWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAVTALNSESNFARAYAQGISRTKYWELIYEDSMDLIAKLPCVAAKIYRNLYREGSGIGAIDSNLDWSHNFTNMLGYTDHQFTELTRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYTCQREFALKHLPNDPMFKLVAQLYKIVPNVLLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTEGLMKFVDSKSG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 citrate synthase
别名 CS, 2F9G6, CISY, Citrate, Citrate synthase, mitochondrial
计算分子量 466 aa, 52 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC010106
基因名称 Citrate Synthase
Gene ID (NCBI) 1431
RRIDAB_2918548
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO75390
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

||
New chat Able
正在加载，请稍候...