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验证数据展示

  • WB analysis using 80017-1-RR (same clone as 80017-1-PBS)

    WB analysis using 80017-1-RR (same clone as 80017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CALR antibody included
  • IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CALR antibody included
  • Affinity and Kinetic Characterization of 80017-1-RR

    Affinity and Kinetic Characterization of 80017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80017-1-RR against Human calreticulin were performed. The affinity constant is 6.07 nM.

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calreticulin Recombinant monoclonal antibody, PBS Only

calreticulin Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80017-1-PBS

产品说明书

CloneNo. 2B17

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

CALR, Calregulin, 2B17

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CALR Ready-To-Use IHC Kit. CALR primary antibody included.

产品信息

80017-1-PBS targets calreticulin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26064

Product name: Recombinant human calreticulin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 248-417 aa of BC002500

Sequence: KKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calreticulin
别名 CALR, Calregulin, 2B17
计算分子量 60 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC002500
基因名称 Calreticulin
Gene ID (NCBI) 811
RRIDAB_2882941
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP27797
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.