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Host
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验证数据展示

  • WB analysis using 84045-1-RR (same clone as 84045-1-PBS)

    WB analysis using 84045-1-RR (same clone as 84045-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84045-1-RR (cGAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 84045-1-RR (same clone as 84045-1-PBS)

    IP experiment of NIH/3T3 using 84045-1-RR (same clone as 84045-1-PBS)

    IP result of anti-cGAS (IP:84045-1-RR, 4ug; Detection:84045-1-RR 1:600) with NIH/3T3 cells lysate 1920 ug. This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 84045-1-RR (same clone as 84045-1-PBS)

    IF Staining of RAW 264.7 using 84045-1-RR (same clone as 84045-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using cGAS antibody (84045-1-RR, Clone: 240964E2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84045-1-RR

    Affinity and Kinetic Characterization of 84045-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84045-1-RR against Mouse cGAS were performed. The affinity constant is below 1 pM.

Mouse cGAS Recombinant antibody, PBS Only

cGAS Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 84045-1-PBS

产品说明书

CloneNo. 240964E2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, IP, ELISA

别名

240964E2, m cGAS, Mb21d1, mcGAS, m-cGAS

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84045-1-PBS targets cGAS in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 mouse
免疫原 cGAS fusion protein Ag31677 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RIKEN cDNA E330016A19 gene
别名 240964E2, m cGAS, Mb21d1, mcGAS, m-cGAS
计算分子量58 kDa
观测分子量58 kDa
GenBank蛋白编号BC145651
基因名称 cGAS
Gene ID (NCBI) 214763
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8C6L5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.