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验证数据展示

  • IHC staining of human placenta using 29958-1-AP (same clone as 29958-1-PBS)

    IHC staining of human placenta using 29958-1-AP (same clone as 29958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 29958-1-AP (same clone as 29958-1-PBS)

    IHC staining of human lung using 29958-1-AP (same clone as 29958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 29958-1-AP (same clone as 29958-1-PBS)

    IF Staining of THP-1 using 29958-1-AP (same clone as 29958-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using cGAS antibody (29958-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

  • WB analysis using 29958-1-AP (same clone as 29958-1-PBS)

    WB analysis using 29958-1-AP (same clone as 29958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29958-1-AP (cGAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Mouse cGAS Polyclonal antibody, PBS Only

cGAS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29958-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

m cGAS, Mb21d1, mcGAS, m-cGAS

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29958-1-PBS targets cGAS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31677

Product name: Recombinant mouse cGAS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 350-507 aa of BC145651

Sequence: KNAKDGNSFQGETWRLSFSHTEKYILNNHGIEKTCCESSGAKCCRKECLKLMKYLLEQLKKEFQELDAFCSYHVKTAIFHMWTQDPQDSQWDPRNLSSCFDKLLAFFLECLRTEKLDHYFIPKFNLFSQELIDRKSKEFLSKKIEYERNNGFPIFDKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RIKEN cDNA E330016A19 gene
别名 m cGAS, Mb21d1, mcGAS, m-cGAS
计算分子量58 kDa
观测分子量58 kDa
GenBank蛋白编号BC145651
基因名称 cGAS
Gene ID (NCBI) 214763
RRIDAB_2935491
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8C6L5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.

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