The mean TGFBI/BIGH3 concentration was determined to be 136.1 ng/mL in HUVEC cell extract based on a 2.2 mg/mL extract load.

HUVEC cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for human TGFBI/BIGH3, and measured 7.9 ng/mL.

Plasma of six individual healthy human donors was measured. The TGFBI/BIGH3 concentration of detected samples was determined to be 1,016.5 ng/mL with a range of 640.9-1,282.5 ng/mL.

Sandwich ELISA standard curve of MP02279-2, Human TGFBI/BIGH3 Recombinant Matched Antibody Pair - PBS only. 80805-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1233. 80805-2-PBS was HRP conjugated as the detection antibody. Range: 97.7-6250 pg/mL.

The mean TGFBI/BIGH3 concentration was determined to be 3586.8 ng/mL in human serum.

Cytometric bead array standard curve of MP02279-1, TGFBI/BIGH3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80805-3-PBS. Detection antibody: 80805-2-PBS. Standard: Eg1233. Range: 0.781-100 ng/mL.

Biolayer interferometry (BLl) kinetic assays of 80805-2-PBS against Human beta IG-H3/TGFBI were performed. The affinity constant is 1.41 nM.