- Featured Product
- KD/KO Validated
smooth muscle actin specific Recombinant antibody
smooth muscle actin specific Recombinant Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat, chicken, pig, zebrafish and More (1)
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
5H7
验证数据展示
经过测试的应用
Positive WB detected in | mouse colon tissue, pig vein tissue, chicken crop tissue, zebrafish tissue, A549 cells, pig stomach, pig colon, rat stomach, chicken small intestine |
Positive IHC detected in | human liver tissue, human heart tissue, human appendicitis tissue, mouse colon tissue, rat colon tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse heart tissue, mouse embryo tissue |
Positive FC detected in | C2C12 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
产品信息
80008-1-RR targets smooth muscle actin specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat, chicken, pig, zebrafish samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | IF, IHC, WB |
Tested Reactivity | Human, mouse, rat, chicken, pig, zebrafish |
Cited Reactivity | human, mouse |
Immunogen | Peptide |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | actin, alpha 2, smooth muscle, aorta |
Synonyms | a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, SMA, smooth muscle actin, smooth muscle actin specific |
Calculated molecular weight | 42 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | NM_001613 |
Gene symbol | ACTA2 |
Uniprot | P62736 |
Gene ID (NCBI) | 59 |
RRID | AB_2882938 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.
实验方案
Product Specific Protocols | |
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WB protocol for smooth muscle actin specific antibody 80008-1-RR | Download protocol |
IHC protocol for smooth muscle actin specific antibody 80008-1-RR | Download protocol |
IF protocol for smooth muscle actin specific antibody 80008-1-RR | Download protocol |
FC protocol for smooth muscle actin specific antibody 80008-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Pharmacol Res MicroRNA-24-3p alleviates cardiac fibrosis by suppressing cardiac fibroblasts mitophagy via downregulating PHB2. | ||
Drug Des Devel Ther Ginsenoside Rb1 Alleviates Bleomycin-Induced Pulmonary Inflammation and Fibrosis by Suppressing Central Nucleotide-Binding Oligomerization-, Leucine-Rich Repeat-, and Pyrin Domains-Containing Protein Three Inflammasome Activation and the NF-κB Pathway. | ||
Exp Cell Res FGF2 isoforms play distinct roles in tubular epithelial-to-mesenchymal transition in diabetic nephropathy |