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验证数据展示

  • IF Staining of mouse embryo using 80008-1-RR

    IF Staining of mouse embryo using 80008-1-RR

    E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

  • WB analysis using 80008-1-RR

    WB analysis using 80008-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 80008-1-RR

    IHC staining of human liver using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • WB analysis using 80008-1-RR

    WB analysis using 80008-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 80008-1-RR

    IHC staining of human colon using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • WB analysis of A549 using 80008-1-RR

    WB analysis of A549 using 80008-1-RR

    WB result of smooth muscle actin specific antibody (80008-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin specific transfected A549 cells.

  • IHC staining of human breast cancer using 80008-1-RR

    IHC staining of human breast cancer using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on ‌an‌ autostainer.

  • IHC staining of human breast cancer using 80008-1-RR

    IHC staining of human breast cancer using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on ‌an‌ autostainer.

  • IHC staining of human ovary cancer using 80008-1-RR

    IHC staining of human ovary cancer using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of human appendicitis using 80008-1-RR

    IHC staining of human appendicitis using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of human breast cancer using 80008-1-RR

    IHC staining of human breast cancer using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of human liver cancer using 80008-1-RR

    IHC staining of human liver cancer using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of rat liver using 80008-1-RR

    IHC staining of rat liver using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of rat colon using 80008-1-RR

    IHC staining of rat colon using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of mouse colon using 80008-1-RR

    IHC staining of mouse colon using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of human rectal cancer using 80008-1-RR

    IHC staining of human rectal cancer using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of human heart using 80008-1-RR

    IHC staining of human heart using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of human stomach cancer using 80008-1-RR

    IHC staining of human stomach cancer using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of human colorectal cancer using 80008-1-RR

    IHC staining of human colorectal cancer using 80008-1-RR

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IF Staining of mouse heart using 80008-1-RR

    IF Staining of mouse heart using 80008-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 80008-1-RR

    IF Staining of mouse small intestine using 80008-1-RR

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using Alpha smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of C2C12 using 80008-1-RR

    FC experiment of C2C12 using 80008-1-RR

    1x10^6 C2C12 cells were intracellularly stained with 0.5 ug smooth muscle actin specific Recombinant antibody (80008-1-RR, Clone:5H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

Alpha smooth muscle actin specific Recombinant monoclonal antibody

Alpha smooth muscle actin specific Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA
Cat No. 80008-1-RR

产品说明书

CloneNo. 5H7
引用文献(85)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig, chicken, zebrafish and More (2)

应用

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA and More (1)

别名

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue, pig vein tissue, chicken crop tissue, zebrafish tissue, A549 cells, pig stomach, pig colon, rat stomach, chicken small intestine tissue, rat colon tissue
Positive IHC detected inhuman breast cancer tissue, human appendicitis tissue, human colon tissue, human colorectal cancer tissue, human heart tissue, human liver tissue, human liver cancer tissue, human ovary cancer tissue, human placenta tissue, human rectal cancer tissue, human stomach cancer tissue, human tonsillitis tissue, mouse colon tissue, rat colon tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue, mouse embryo tissue
Positive IF-Fro detected inmouse small intestine tissue
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80008-1-RR targets Alpha smooth muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.

经测试应用 WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig, chicken, zebrafish
文献引用反应性human, mouse, rat, pig, rabbit, duck
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actin, alpha 2, smooth muscle, aorta
别名 ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA
计算分子量 42 kDa
观测分子量42 kDa
GenBank蛋白编号NM_001613
基因名称 Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_2882938
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62736
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

实验方案

Product Specific Protocols
FC protocol for Alpha smooth muscle actin specific antibody 80008-1-RRDownload protocol
IF protocol for Alpha smooth muscle actin specific antibody 80008-1-RRDownload protocol
IHC protocol for Alpha smooth muscle actin specific antibody 80008-1-RRDownload protocol
WB protocol for Alpha smooth muscle actin specific antibody 80008-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Gastroenterology

Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells

Authors - Decai Wang
View Article

Adv Mater

Reprogramming the Immunosuppressive Microenvironment in Triple-Negative Breast Cancer via FAP-Targeted Nanoprobes for Multimodal Imaging and Photothermal Therapy.

Authors - Ling Zhan
View Article
mouseIF

Nat Genet

Single-cell multi-omic and spatial profiling of esophageal squamous cell carcinoma reveals the immunosuppressive role of GPR116+ pericytes in cancer metastasis

Authors - Xiaoya Pei
View Article
humanWB,IF

Cancer Res

YAP1 Inhibition Induces Phenotype Switching of Cancer-Associated Fibroblasts to Tumor Suppressive in Prostate Cancer

Authors - Hongtao Song
View Article
mouseWB

Autophagy

Autophagic degradation of SQSTM1 enables fibroblast activation to accelerate wound healing

Authors - Yujiao Xu
View Article
IF

Nat Protoc

Bioink design for organ-scale projection-based 3D bioprinting

Authors - Tianhong Qiao
View Article
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