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smooth muscle actin specific Recombinant antibody

smooth muscle actin specific Recombinant Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, chicken, pig, zebrafish and More (1)

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5H7

Cat No : 80008-1-RR

Print datasheet

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, SMA, smooth muscle actin, smooth muscle actin specific



经过测试的应用

Positive WB detected inmouse colon tissue, pig vein tissue, chicken crop tissue, zebrafish tissue, A549 cells, pig stomach, pig colon, rat stomach, chicken small intestine
Positive IHC detected inhuman liver tissue, human heart tissue, human appendicitis tissue, mouse colon tissue, rat colon tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, mouse embryo tissue
Positive FC detected inC2C12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

80008-1-RR targets smooth muscle actin specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat, chicken, pig, zebrafish samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity Human, mouse, rat, chicken, pig, zebrafish
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name actin, alpha 2, smooth muscle, aorta
Synonyms a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, SMA, smooth muscle actin, smooth muscle actin specific
Calculated molecular weight 42 kDa
Observed molecular weight42 kDa
GenBank accession numberNM_001613
Gene symbol ACTA2
UniprotP62736
Gene ID (NCBI) 59
RRIDAB_2882938
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

实验方案

Product Specific Protocols
WB protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
IHC protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
IF protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
FC protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Pharmacol Res

MicroRNA-24-3p alleviates cardiac fibrosis by suppressing cardiac fibroblasts mitophagy via downregulating PHB2.

Authors - Yue Zhang
mouseWB,IHC,IF

Drug Des Devel Ther

Ginsenoside Rb1 Alleviates Bleomycin-Induced Pulmonary Inflammation and Fibrosis by Suppressing Central Nucleotide-Binding Oligomerization-, Leucine-Rich Repeat-, and Pyrin Domains-Containing Protein Three Inflammasome Activation and the NF-κB Pathway.

Authors - Jingjing Liu
human,mouseWB

Exp Cell Res

FGF2 isoforms play distinct roles in tubular epithelial-to-mesenchymal transition in diabetic nephropathy

Authors - Yingying Luo