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验证数据展示

  • WB analysis using 86336-1-RR

    WB analysis using 86336-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86336-1-RR (ZnT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 86336-1-RR

    IHC staining of human prostate cancer using 86336-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86336-1-RR (ZnT4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86336-1-RR

    Affinity and Kinetic Characterization of 86336-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86336-1-RR against Human ZnT4 were performed. The affinity constant is 3.87 nM.

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ZnT4 Recombinant monoclonal antibody

ZnT4 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86336-1-RR

产品说明书

CloneNo. 250909D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

SLC30A4, Probable proton-coupled zinc antiporter SLC30A4, ZnT 4, ZnT-4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, mouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86336-1-RR targets ZnT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3313

Product name: Recombinant human SLC30A4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-120 aa of BC026089

Sequence: MAGSGAWKRLKSMLRKDDAPLFLNDTSAFDFSDEAGDEGLSRFNKLRVVVADDGSEAPERPVNGAHPTLQADDDSLLDQDLPLTNSQLSLKVDSCDNCSKQREILKQRKVKARLTIAAVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 30 (zinc transporter), member 4
别名 SLC30A4, Probable proton-coupled zinc antiporter SLC30A4, ZnT 4, ZnT-4
计算分子量 429 aa, 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC026089
基因名称 ZNT4
Gene ID (NCBI) 7782
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14863
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZnT4, also known as SLC30A4, belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. ZnT4 transports Zn into the trans-Golgi apparatus for lactose synthesis, and across the apical cell membrane for efflux from MECs into milk. ZnT4 is expressed in numerous tissues and is enriched in the prostate. ZnT4 encodes a protein with a molecular weight of 47 kDa (PMID: 26538236).

实验方案

Product Specific Protocols
WB protocol for ZnT4 antibody 86336-1-RRDownload protocol
IHC protocol for ZnT4 antibody 86336-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols