验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse liver tissue, mouse heart tissue, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33605-1-AP targets ZZEF1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag35406 Product name: Recombinant human ZZEF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2480-2600 aa of BC151836 Sequence: ILRAIYELQMKKTDYFFLEVQKRFDGDELTTDERIRSLAQRWQPSKSLRLEEQSAKAVDTDMIILPCLSRPARCDQATAESNPVTQKLISSTESELQQSYAKQRRSKSAALLHKELNCKSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, ZZ-type with EF-hand domain 1 |
| 别名 | KIAA0399, Zinc finger ZZ-type and EF-hand domain-containing protein 1 |
| 观测分子量 | 300-330 kDa |
| GenBank蛋白编号 | BC151836 |
| 基因名称 | ZZEF1 |
| Gene ID (NCBI) | 23140 |
| RRID | AB_3743027 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O43149 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZZEF1 is a large cytoplasmic protein distinguished by a ZZ-type zinc finger and an EF-hand calcium-binding domain, hinting at a role in linking protein-protein interactions with calcium-mediated signaling. Its functions are not fully elucidated, but growing evidence suggests involvement in cilia formation, cytoskeletal organization, and cell signaling regulation. Mutations or dysregulation of ZZEF1 have been linked to ciliopathies, tumor biology, and possible neurodevelopmental conditions, making it a gene of emerging biomedical interest. (PMID: 33227311)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZZEF1 antibody 33605-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
