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ZWINT Polyclonal antibody

ZWINT Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12282-2-AP

Print datasheet

Synonyms

HZwint 1, KNTC2AP, ZW10 interacting protein 1, ZW10 interactor, ZWINT, Zwint 1, ZWINT1

验证数据展示

  • WB analysis of HepG2 using 12282-2-AP

    WB analysis of HepG2 using 12282-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12282-2-AP (ZWINT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 12282-2-AP

    FC experiment of HeLa using 12282-2-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZWINT (12282-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12282-2-AP targets ZWINT in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, WB, ELISA
Tested Reactivity human
Immunogen ZWINT fusion protein Ag2933 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ZW10 interactor
Synonyms HZwint 1, KNTC2AP, ZW10 interacting protein 1, ZW10 interactor, ZWINT, Zwint 1, ZWINT1
Calculated Molecular Weight 277 aa, 31 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC020979
Gene Symbol ZWINT
Gene ID (NCBI) 11130
RRIDAB_2221166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZWINT antibody 12282-2-APDownload protocol
FC protocol for ZWINT antibody 12282-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols