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验证数据展示

  • WB analysis using 24561-1-AP (same clone as 24561-1-PBS)

    WB analysis using 24561-1-AP (same clone as 24561-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 24561-1-AP (same clone as 24561-1-PBS)

    IP experiment of Jurkat using 24561-1-AP (same clone as 24561-1-PBS)

    IP result of anti-ZW10 (IP:24561-1-AP, 4ug; Detection:24561-1-AP 1:500) with Jurkat cells lysate 3200ug. This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 24561-1-AP (same clone as 24561-1-PBS)

    FC experiment of HeLa using 24561-1-AP (same clone as 24561-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZW10 (24561-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

  • WB analysis using 24561-1-AP (same clone as 24561-1-PBS)

    WB analysis using 24561-1-AP (same clone as 24561-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 24561-1-AP (same clone as 24561-1-PBS)

    WB analysis of HeLa using 24561-1-AP (same clone as 24561-1-PBS)

    WB result of ZW10 antibody (24561-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZW10 transfected HeLa cells. This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 24561-1-AP (same clone as 24561-1-PBS)

    IF Staining of HeLa using 24561-1-AP (same clone as 24561-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ZW10 antibody (24561-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZW10 Polyclonal antibody, PBS Only

ZW10 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 24561-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), IP, ELISA

别名

HZW10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ZW10 Ready-To-Use IHC Kit. ZW10 primary antibody included.

产品信息

24561-1-PBS targets ZW10 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19138

Product name: Recombinant human ZW10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 318-672 aa of BC128264

Sequence: NEKTSTVPLAEMLGDMIWEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIHNTVKIIPDSKINVPELPTPDEDNKLEVQKVSNTQYHEVMNLEPENTLDQHSFSLPTCRISESVKKLMELAYQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRLRLAPILCDGTATFVDLVPGFRRLGTECFLAQMRAQKGELLERLSSARNFSNMDDEENYSAASKAVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTAISEVIGKITALE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ZW10, kinetochore associated, homolog (Drosophila)
别名 HZW10
计算分子量 779 aa, 89 kDa
观测分子量 88-90 kDa
GenBank蛋白编号BC128264
基因名称 ZW10
Gene ID (NCBI) 9183
RRIDAB_2815001
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43264
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZW10 is the human homolog of the Drosophila melanogaster Zw10 protein. ZW10 is localized to kinetochores and kinetochore-associated microtubules and is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis(PMID: 17102640). ZW10 proteins in Drosophila and HeLa cells display a similar and intriguing cell cycle-dependent intracellular distribution. ZW10 protein first becomes localized to the kinetochore at prometaphase, but then appears to move onto the kinetochore microtubules of the spindle at metaphase, and then back to the kinetochore at anaphase(PMID: 9700164).

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