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验证数据展示

  • WB analysis using 21630-1-AP

    WB analysis using 21630-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21630-1-AP (ZSCAN29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 21630-1-AP

    IHC staining of human colon using 21630-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21630-1-AP (ZSCAN29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 21630-1-AP

    IHC staining of human colon using 21630-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21630-1-AP (ZSCAN29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZSCAN29 Polyclonal antibody

ZSCAN29 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21630-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Zfp690, Zinc finger protein 690, ZNF690, ZSCAN29

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21630-1-AP targets ZSCAN29 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ZSCAN29 fusion protein Ag16239 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and SCAN domain containing 29
别名 Zfp690, Zinc finger protein 690, ZNF690, ZSCAN29
计算分子量 852 aa, 97 kDa
观测分子量96-97 kDa
GenBank蛋白编号BC109271
基因名称 ZSCAN29
Gene ID (NCBI) 146050
RRIDAB_3085664
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZSCAN29 (zinc finger and SCAN domain containing 29), also named as ZNF690 or Zfp690. It is predicted to be located in nucleus and ubiquitous expression in testis and lymph node. The calculated molecular weight of ZSCAN29 is 96 kDa. The genes ZSCAN29 (associated with five disorders: SCZ, BIP, MD, ASD and ADHD) and ZSCAN31 (associated with three disorders: SCZ, BIP and MD) encode zing finger transcription factor proteins that belong to the krueppel C2H2-type zinc-finger protein family. Zinc finger proteins are essential in multiple regulatory mechanisms and some have been associated with psychiatric disorders as reviewed by Squassina et al., 2019 (PMID: 34637873). It also enables sequence-specific double-stranded DNA binding activity and be predicted to be involved in regulation of transcription by RNA polymerase II.

实验方案

Product Specific Protocols
WB protocol for ZSCAN29 antibody 21630-1-APDownload protocol
IHC protocol for ZSCAN29 antibody 21630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols