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验证数据展示

  • WB analysis using 20855-1-AP

    WB analysis using 20855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 20855-1-AP

    IHC staining of human small intestine using 20855-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:400.

ZSCAN20 Polyclonal antibody

ZSCAN20 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20855-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KOX29, ZFP 31, Zinc finger and SCAN domain-containing protein 20, ZNF31, ZNF360

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, MG-63 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20855-1-AP targets ZSCAN20 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14798

Product name: Recombinant human ZSCAN20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 54-400 aa of BC008827

Sequence: EEAVALVEDWHRETRTAGQSGLELHTEETRPLKTGEEAQSFQLQPVDPWPEGQSQKKGVKNTCPDLPNHLNAEVAPQPLKESAVLTPRVPTLPKMGSVGDWEVTAESQEALGPGKHAEKELCKDPPGDDCGNSVCLGVPVSKPSNTSEKEQGPEFWGLSLINSGKRSTADYSLDNEPAQALTWRDSRAWEEQYQWDVEDMKVSGVHWGYEETKTFLAILSESPFSEKLRTCHQNRQVYRAIAEQLRARGFLRTLEQCRYRVKNLLRNYRKAKSSHPPGTCPFYEELEALVRARTAIRATDGPGEAVALPRLGDSDAEMDEQEEGGWDPEEMAEDCNGAGLVNVESTQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and SCAN domain containing 20
别名 KOX29, ZFP 31, Zinc finger and SCAN domain-containing protein 20, ZNF31, ZNF360
计算分子量 1043 aa, 118 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC008827
基因名称 ZSCAN20
Gene ID (NCBI) 7579
RRIDAB_2878753
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17040
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ZSCAN20 antibody 20855-1-APDownload protocol
WB protocol for ZSCAN20 antibody 20855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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