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验证数据展示

  • WB analysis of HepG2 using 20855-1-AP

    WB analysis of HepG2 using 20855-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 20855-1-AP

    IHC staining of human small intestine using 20855-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:400.

ZSCAN20 Polyclonal antibody

ZSCAN20 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20855-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KOX29, ZFP 31, Zinc finger protein 31, Zinc finger protein 360, Zinc finger protein KOX29, ZNF31, ZNF360, ZSCAN20

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20855-1-AP targets ZSCAN20 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14798

Product name: Recombinant human ZSCAN20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 54-400 aa of BC008827

Sequence: EEAVALVEDWHRETRTAGQSGLELHTEETRPLKTGEEAQSFQLQPVDPWPEGQSQKKGVKNTCPDLPNHLNAEVAPQPLKESAVLTPRVPTLPKMGSVGDWEVTAESQEALGPGKHAEKELCKDPPGDDCGNSVCLGVPVSKPSNTSEKEQGPEFWGLSLINSGKRSTADYSLDNEPAQALTWRDSRAWEEQYQWDVEDMKVSGVHWGYEETKTFLAILSESPFSEKLRTCHQNRQVYRAIAEQLRARGFLRTLEQCRYRVKNLLRNYRKAKSSHPPGTCPFYEELEALVRARTAIRATDGPGEAVALPRLGDSDAEMDEQEEGGWDPEEMAEDCNGAGLVNVESTQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and SCAN domain containing 20
别名 KOX29, ZFP 31, Zinc finger protein 31, Zinc finger protein 360, Zinc finger protein KOX29, ZNF31, ZNF360, ZSCAN20
计算分子量 1043 aa, 118 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC008827
基因名称 ZSCAN20
Gene ID (NCBI) 7579
RRIDAB_2878753
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17040
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ZSCAN20 antibody 20855-1-APDownload protocol
WB protocol for ZSCAN20 antibody 20855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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