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验证数据展示

  • WB analysis using 32581-1-AP

    WB analysis using 32581-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32581-1-AP (ZSCAN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ZSCAN2 Polyclonal antibody

ZSCAN2 Polyclonal Antibody for WB, ELISA
Cat No. 32581-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ZNF854, Zinc finger protein 854, Zinc finger protein 29 homolog, Zinc finger and SCAN domain-containing protein 2, Zfp-29

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32581-1-AP targets ZSCAN2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ZSCAN2 fusion protein Ag38126 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and SCAN domain containing 2
别名 ZNF854, Zinc finger protein 854, Zinc finger protein 29 homolog, Zinc finger and SCAN domain-containing protein 2, Zfp-29
计算分子量70 kDa
观测分子量70 kDa
GenBank蛋白编号BC136342
基因名称 ZSCAN2
Gene ID (NCBI) 54993
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ7Z7L9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc finger and SCAN domain-containing protein 2 (ZSCAN2, also known as ZFP29 and ZNF854) plays an important role in the maintenance of the SSC (spermatogonial stem cells) pool in vitro and is integral for robust regeneration of spermatogenesis in vivo following exposure to clastogens. Loss of ZSCAN2 expression alters key cellular processes in undifferentiated spermatogonia, such as translation, chromatin modification, and ubiquitin conjugation (PMID: 35244684).

实验方案

Product Specific Protocols
WB protocol for ZSCAN2 antibody 32581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols