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验证数据展示

  • IHC staining of human stomach using 30704-1-AP

    IHC staining of human stomach using 30704-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30704-1-AP (ZSCAN18 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach using 30704-1-AP

    IHC staining of human stomach using 30704-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30704-1-AP (ZSCAN18 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 30704-1-AP

    IF Staining of SH-SY5Y using 30704-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using ZSCAN18 antibody (30704-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ZSCAN18 Polyclonal antibody

ZSCAN18 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30704-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

ZSCAN 18

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30704-1-AP targets ZSCAN18 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33264

Product name: Recombinant human ZSCAN18 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 256-510 aa of BC022846

Sequence: DAASRLDTEELRLVERDPQGSSLPEGGRRQESAGCACEEAAPAGVLPELPTEAPPGDALADPPSGTTEEEEEQPGKAPDPQDPQDAESDSATGSQRQSVIQQPAPDRGTAKLGTKRPHPEDGDEQSLEGVSSSGDSAGLEAGQGPGADEPGLSRGKPYACGECGEAFAWLSHLMEHHSSHGGRKRYACQGCWKTFHFSLALAEHQKTHEKEKSYALGGARGPQPSTREAQAGARAGGPPESVEGEAPPAPPEAQR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and SCAN domain containing 18
别名 ZSCAN 18
计算分子量 510 aa, 55 kDa
GenBank蛋白编号BC022846
基因名称 ZSCAN18
Gene ID (NCBI) 65982
RRIDAB_3742368
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TBC5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc finger and scan domain containing 18 (ZSCAN18) is a ZSCAN TF (a subfamily of ZNF TFs). ZSCAN18 is also known as ZNF447 and is located in chromosome 19q13.43. Recently, hypermethylation in the promoter of ZSCAN18 has been identified in various types of cancers, such as esophageal cancer , renal cell carcinoma, cholangiocarcinoma , colorectal cancer , GC , hepatocellular carcinoma , and pancreatic cancer .(PMID: 36650573, PMID: 40555855)

实验方案

Product Specific Protocols
IF protocol for ZSCAN18 antibody 30704-1-APDownload protocol
IHC protocol for ZSCAN18 antibody 30704-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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