验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
24816-1-AP targets ZRANB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag20487 Product name: Recombinant human ZRANB2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-241 aa of BC039814 Sequence: MSTKNFRVSDGDWICPDKKCGNVNFARRTSCNRCGREKTTEAKMMKAGGTEIGKTLAEKSRGLFSANDWQCKTCSNVNWARRSECNMCNTPKYAKLEERTGYGGGFNERENVEYIEREESDGEYDEFGRKKKKYRGKAVGPASILKEVEDKESEGEEEDEDEDLSKYKLDEDEDEDDADLSKYNLDASEEEDSNKKKSNRRSRSKSGSSHSRSSSRSSSPSSSRSRSRSRSRSSSSSQSRS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, RAN-binding domain containing 2 |
| 别名 | ZIS2, ZIS1, ZIS, Zinc finger, splicing, Zinc finger Ran-binding domain-containing protein 2 |
| 计算分子量 | 330 aa, 37 kDa |
| 观测分子量 | 37 kDa, 48 kDa |
| GenBank蛋白编号 | BC039814 |
| 基因名称 | ZRANB2 |
| Gene ID (NCBI) | 9406 |
| RRID | AB_2879740 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95218 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZRANB2, also named as ZIS and ZNF265, is a splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. It may interfere with constitutive 5'-splice site selection. The calculated MW of ZRANB2 is 330aa, 37 kDa or 320aa, 36 kDa. We always got the ~48 kDa in our detection. The immunogen of this antibody is 1-241aa. No other protein can be recognized by this antibody from the sequence BLAST results.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZRANB2 antibody 24816-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res ZRANB2 and SYF2-mediated splicing programs converging on ECT2 are involved in breast cancer cell resistance to doxorubicin. | ||
Aging (Albany NY) LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2. | ||
J Exp Clin Cancer Res ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma.
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