验证数据展示
经过测试的应用
| Positive IP detected in | HeLa cells, mouse cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
27050-1-AP targets ZRANB1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag25789 Product name: Recombinant human ZRANB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of AL832925 Sequence: MSERGIKWACEYCTYENWPSAIKCTMCRAQRPSGTIITEDPFKSGSSDVGRDWDPSSTEGGSSPLICPDSSARPRVKSSYSMENANKWSCHMCTYLNWPR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, RAN-binding domain containing 1 |
| 别名 | hTrabid, TRABID, Ubiquitin thioesterase ZRANB1 |
| 计算分子量 | 81 kDa |
| 观测分子量 | 81 kDa |
| GenBank蛋白编号 | AL832925 |
| 基因名称 | ZRANB1 |
| Gene ID (NCBI) | 54764 |
| RRID | AB_3742213 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UGI0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZRANB1 is an 81-kDa deubiquitinating enzyme with an Npl14 zinc finger (NZF) domain at the N-terminus and an ovarian tumor (OTU) domain at the C-terminus. ZRANB1 specifically cleaves K29-, K33-, and K63-linked ubiquitin chains, but a mutation of amino acid 443 from cysteine to serine ablates enzymatic activity . (PMID: 36087511, PMID: 34765294)
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ZRANB1 antibody 27050-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
