验证数据展示
经过测试的应用
| Positive IP detected in | mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
15835-1-AP targets ZPBP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag8585 Product name: Recombinant human ZPBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC005223 Sequence: MEAFALGPARRGRRRTRAAGSLLSRAAILLFISAFLVRVPSSVGHLVRLPRAFRLTKDSVKIVGSTSFPVKAYVMLHQKSPHVLCVTQQLRNAELIDPSFQWYGPKGKVVSVENRTAQITSTGSLVFQNFEESMSGIYTCFLEYKPTVEEIVKRLQLKYAIYAYREPHYYYQFTARYHAAPCNSIYNISFEKKLLQILSKLLLDLSCEISLLKSECHRVKMQRAGLQNELFFAFSVSSLDTEKGPKRCTDHNCEPYKRLFKAKNLIERFFNQQVEILGRRAEQLPQIYYIEGTLQMVWINRCFPGYGMNVQQHPKCPECCVICSPGSYNPRDGIHCLQCNSSLVYGAKTCL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zona pellucida binding protein |
| 别名 | SP38, zona pellucida binding protein, ZPBP, ZPBP1 |
| 计算分子量 | 351 aa, 40 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC005223 |
| 基因名称 | ZPBP |
| Gene ID (NCBI) | 11055 |
| RRID | AB_2878189 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BS86 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZPBP (also known as ZPBP1), named for its binding to the oocyte zona pellucida, is a spermatid and spermatozoon protein that localizes to the acrosome. ZPBP may be implicated in gamete interaction during fertilization. It has been reported that absence of ZPBP1 prevents acrosome compaction and leads to acrosome fragmentation and sterility in male mice. (PMID: 17664285; 19204925)
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ZPBP antibody 15835-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
