×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 21832-1-AP (same clone as 21832-1-PBS)

    WB analysis using 21832-1-AP (same clone as 21832-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21832-1-AP (ZP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21832-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse ovary using 21832-1-AP (same clone as 21832-1-PBS)

    IF Staining of mouse ovary using 21832-1-AP (same clone as 21832-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP2 antibody (21832-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21832-1-PBS in a different storage buffer formulation.

ZP2 Polyclonal antibody, PBS Only

ZP2 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 21832-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

21832-1-PBS targets ZP2 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16386

Product name: Recombinant human ZP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 440-745 aa of BC096304

Sequence: EIHALWTDFPPSKISRDSEFRMTVKCSYSRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPYGENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQWNVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSSLVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGPILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTAGDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zona pellucida glycoprotein 2 (sperm receptor)
别名
计算分子量 745 aa, 82 kDa
观测分子量75-82 kDa
GenBank蛋白编号BC096304
基因名称 ZP2
Gene ID (NCBI) 7783
RRIDAB_3669375
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05996
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZP2 is a major component of the zona pellucida to which sperm bind prior to penetrating the zona matrix and fusing with the egg. Following fertilization and cortical granule exocytosis, ZP2 is cleaved by ovastacin after which sperm do not bind to the zona pellucida. The zona pellucida and ovastacin proteins transverse the endomembrane system during oogenesis during which passage ZP2 remains intact. Secreted ZP2 is incorporated into the extracellular zona pellucida surrounding ovulated eggs and is cleaved in the zona matrix surrounding 2-cell embryos. (PMID: 28114310)

||
New chat

Able™

正在加载，请稍候...