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验证数据展示

  • WB analysis using RMX00002

    WB analysis using RMX00002

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00002 (ZO1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using RMX00002

    IP experiment of HEK-293 using RMX00002

    IP result of anti-ZO1 (IP:RMX00002, 4ug; Detection:RMX00002 1:20000) with HEK-293 cells lysate 1800 ug.

  • IHC staining of mouse colon using RMX00002

    IHC staining of mouse colon using RMX00002

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat colon using RMX00002

    IHC staining of rat colon using RMX00002

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat colon using RMX00002

    IHC staining of rat colon using RMX00002

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using RMX00002

    IF Staining of MCF-7 using RMX00002

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (RMX00002) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Multi-rAb™ ZO-1 Multi-Recombinant antibody

ZO-1 Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. RMX00002

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

TJP1, ZO1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HEK-293 cells, HT-29 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inrat colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00002 targets ZO-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 tight junction protein 1 (zona occludens 1)
别名 TJP1, ZO1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1
计算分子量 1748 aa, 195 kDa
观测分子量 230 kDa
GenBank蛋白编号BC111712
基因名称 ZO-1
Gene ID (NCBI) 7082
偶联类型 Unconjugated
形式 Liquid
纯化方式N/A
UNIPROT IDQ07157
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tight junction (or zonula occludens) forms the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).

实验方案

Product Specific Protocols
WB protocol for ZO-1 antibody RMX00002Download protocol
IHC protocol for ZO-1 antibody RMX00002Download protocol
IF protocol for ZO-1 antibody RMX00002Download protocol
IP protocol for ZO-1 antibody RMX00002Download protocol
Standard Protocols
Click here to view our Standard Protocols