验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, HCT 116 cells, Caco-2 cells |
| Positive IP detected in | HT-29 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87266-1-RR targets ZO-3 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg5585 Product name: Recombinant human ZO3/Zonula occludens PROTEIN 3 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 835-919 aa of NM_001267560.1 Sequence: DEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tight junction protein 3 (zona occludens 3) |
| 别名 | Tight junction protein 3, Tight junction protein ZO-3, TJP3, ZO3, Zona occludens protein 3 |
| 计算分子量 | 101 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | NM_001267560.1 |
| 基因名称 | ZO-3 |
| Gene ID (NCBI) | 27134 |
| RRID | AB_3745435 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95049-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tight junction (or zonula occludens) forms the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-3 (also known as TJP3)is a key scaffold protein of tight junctions. ZO-3 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs).ZO-3 regulates the stability of Cyclin D1, thereby influencing the proliferation of epithelial cells (PMID: 21411630).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ZO-3 antibody 87266-1-RR | Download protocol |
| WB protocol for ZO-3 antibody 87266-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
