• Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ZO-1 Monoclonal antibody

ZO-1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66452

产品说明书

CloneNo. 1G4A1

宿主/亚型

Mouse / IgG1

种属反应性

human, canine

应用

IF/ICC

TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66452-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66452 targets ZO-1 in IF/ICC applications and shows reactivity with human, canine samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, canine
免疫原 ZO-1 fusion protein Ag16454 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tight junction protein 1 (zona occludens 1)
别名 TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1
计算分子量 1748 aa, 195 kDa
观测分子量 230 kDa
GenBank蛋白编号BC111712
基因名称 ZO-1
Gene ID (NCBI) 7082
RRIDAB_2919329
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ07157
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ZO-1 antibody CL488-66452Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...