验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, mouse brain tissue, mouse testis tissue, mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
20200-1-AP targets ZNRF2-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
fusion protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc and ring finger 2 |
| 别名 | Protein Ells2, RING finger protein 202, RNF202, zinc and ring finger 2, Zinc/RING finger protein 2, ZNRF2, ZNRF2-Specific |
| 计算分子量 | 24 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | NM_147128 |
| 基因名称 | ZNRF2 |
| Gene ID (NCBI) | 223082 |
| RRID | AB_2878657 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NHG8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNRF2 also named as RNF202, may play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. The antibody is specific to ZNRF2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNRF2-Specific antibody 20200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Neurol ZNRF2 attenuates focal cerebral ischemia/reperfusion injury in rats by inhibiting mTORC1-mediated autophagy. | ||
Mol Neurobiol Deletion of ZNRF2 Exacerbates MPTP-Induced Parkinson's Disease by Activating mTOR-Mediated Neuroinflammatory Pathways | ||
Hum Cell ZNRF2 as an oncogene is transcriptionally regulated by CREB1 in breast cancer models | ||
J Cell Mol Med A pancancer analysis of the oncogenic role of ZNRF2 in human tumours
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