验证数据展示
经过测试的应用
| Positive WB detected in | hTERT-RPE1 cells, Jurkat cells, T-47D cells, MOLT-4 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68331-1-Ig targets ZNHIT3 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag29872 Product name: Recombinant human ZNHIT3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-155 aa of BC017931 Sequence: MASLKCSTVVCVICLEKPKYRCPACRVPYCSVVCFRKHKEQCNPETRPVEKKIRSALPTKTVKPVENKDDDDSIADFLNSDEEEDRVSLQNLKNLGESATLRSLLLNPHLRQLMVNLDQGEDKAKLMRAYMQEPLFVEFADCCLGIVEPSQNEES 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, HIT type 3 |
| 别名 | HNF 4a coactivator, TR interacting protein 3, TRIP 3, TRIP3, zinc finger, HIT type 3, ZNHIT3 |
| 计算分子量 | 155 aa, 18 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC017931 |
| 基因名称 | ZNHIT3 |
| Gene ID (NCBI) | 9326 |
| RRID | AB_2935403 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15649 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNHIT3, also named TRIP3, belongs to the zinc finger HIT (Zf-HIT) domain-containing proteins family. ZNHIT3 encodes a nuclear zinc finger protein previously implicated in transcriptional regulation and small nucleolar ribonucleoprotein particle assembly and thus possibly to pre-ribosomal RNA processing (PMID: 28335020). ZNHIT3 contains at least two domains: a ZN-HIT domain that consists of a double zinc-finger, probably involved in protein−protein interaction, and a PAC-HIT domain that folds into a clamp able to trap an α-helix, here again of about 20 residues, located in the sequence of NUFIP1 (PMID: 35315277). ZNHIT3 has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ZNHIT3 antibody 68331-1-Ig | Download protocol |
| WB protocol for ZNHIT3 antibody 68331-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
