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验证数据展示

  • WB analysis using 16595-1-AP (same clone as 16595-1-PBS)

    WB analysis using 16595-1-AP (same clone as 16595-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16595-1-AP (ZNHIT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 16595-1-AP (same clone as 16595-1-PBS)

    IHC staining of mouse liver using 16595-1-AP (same clone as 16595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16595-1-AP (ZNHIT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZNHIT1 antibody included
  • IHC staining of mouse liver using 16595-1-AP (same clone as 16595-1-PBS)

    IHC staining of mouse liver using 16595-1-AP (same clone as 16595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16595-1-AP (ZNHIT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZNHIT1 antibody included
  • IF Staining of A431 using 16595-1-AP (same clone as 16595-1-PBS)

    IF Staining of A431 using 16595-1-AP (same clone as 16595-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNHIT1 antibody (16595-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16595-1-AP (same clone as 16595-1-PBS)

    IF Staining of HepG2 using 16595-1-AP (same clone as 16595-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNHIT1 antibody (16595-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

ZNHIT1 Polyclonal antibody, PBS Only

ZNHIT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16595-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ZNHIT1 Ready-To-Use IHC Kit. ZNHIT1 primary antibody included.

产品信息

16595-1-PBS targets ZNHIT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9741

Product name: Recombinant human ZNHIT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-154 aa of BC017333

Sequence: MVEKKTSVRSQDPGQRRVLDRAARQRRINRQLEALENDNFQDDPHAGLPQLGKRLPQFDDDADTGKKKKKTRGDHFKLRFRKNFQALLEEQNLSVAEGPNYLTACAGPPSRPQRPFCAVCGFPSPYTCVSCGARYCTVRCLGTHQETRCLKWTV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, HIT type 1
别名
计算分子量 154 aa, 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC017333
基因名称 ZNHIT1
Gene ID (NCBI) 10467
RRIDAB_2220382
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43257
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZNHIT1(Zinc finger HIT domain containing protein 1), aslo named CGBP1 (Cyclin G1 binding protein 1), is a subunit of the SNF-2 related CBP activator protein (SRCAP) complex, which can remodel chromatin by incorporating the histone variant H2A.Z into nucleosomes(PMID: 15647280). ZNHIT1 also can bind to myogenin promoter and mediate H2A.Z incorporation for its expression(PMID: 20473270). ZNHIT1 overexpression induces apoptosis and represses proliferation and invasion of breast cancer cells(PMID: 30928405).

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