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验证数据展示

  • WB analysis of Jurkat using 26315-1-AP

    WB analysis of Jurkat using 26315-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26315-1-AP (ZNF828 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 26315-1-AP

    IP experiment of Jurkat using 26315-1-AP

    IP result of anti-ZNF828 (IP:26315-1-AP, 4ug; Detection:26315-1-AP 1:1000) with Jurkat cells lysate 4000ug.

  • IF Staining of HeLa using 26315-1-AP

    IF Staining of HeLa using 26315-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26315-1-AP (ZNF828 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human intrahepatic cholangiocarcinoma using 26315-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26315-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26315-1-AP (ZNF828 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 26315-1-AP

    IF Staining of HeLa using 26315-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZNF828 antibody (26315-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ZNF828 Polyclonal antibody

ZNF828 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26315-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Chromosome alignment-maintaining phosphoprotein 1, CHAMP1, CHAMP, CAMP, C13orf8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26315-1-AP targets ZNF828 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23698

Product name: Recombinant human ZNF828 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 459-812 aa of BC065237

Sequence: SPASLDFPESQKSSRGGSPDLWKSSFFIEPQKPVFPETRKPGPSGPSESPKAASDIWKPVLSIDTEPRKPALFPEPAKTAPPASPEARKRALFPEPRKHALFPELPKSALFSESQKAVELGDELQIDAIDDQKCDILVQEELLASPKKLLEDTLFPSSKKLKKDNQESSDAELSSSEYIKTDLDAMDIKGQESSSDQEQVDVESIDFSKENKMDMTSPEQSRNVLQFTEEKEAFISEEEIAKYMKRGKGKYYCKICCCRAMKKGAVLHHLVNKHNVHSPYKCTICGKAFLLESLLKNHVAAHGQSLLKCPRCNFESNFPRGFKKHLTHCQSRHNEEANKKLMEALEPPLEEQQI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 828
别名 Chromosome alignment-maintaining phosphoprotein 1, CHAMP1, CHAMP, CAMP, C13orf8
观测分子量 110-130 kDa
GenBank蛋白编号BC065237
基因名称 ZNF828
Gene ID (NCBI) 283489
RRIDAB_2880476
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JM3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF828 antibody 26315-1-APDownload protocol
IHC protocol for ZNF828 antibody 26315-1-APDownload protocol
IF protocol for ZNF828 antibody 26315-1-APDownload protocol
IP protocol for ZNF828 antibody 26315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols