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验证数据展示

  • WB analysis using 17448-1-AP

    WB analysis using 17448-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17448-1-AP (ZNF8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 17448-1-AP

    IHC staining of mouse cerebellum using 17448-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17448-1-AP (ZNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 17448-1-AP

    IF Staining of mouse cerebellum using 17448-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 17448-1-AP

    IF Staining of mouse cerebellum using 17448-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ZNF8 Polyclonal antibody

ZNF8 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 17448-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17448-1-AP targets ZNF8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 ZNF8 fusion protein Ag11571 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 8
别名
计算分子量 575 aa, 65 kDa
观测分子量65 kDa
GenBank蛋白编号BC039323
基因名称 ZNF8
Gene ID (NCBI) 7554
RRIDAB_3669275
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17098
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF8 is a transcriptional repressor. It may modulate BMP and TGF-beta signal transduction, through its interaction with SMAD proteins.

实验方案

Product Specific Protocols
IHC protocol for ZNF8 antibody 17448-1-APDownload protocol
IF protocol for ZNF8 antibody 17448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols