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验证数据展示

  • WB analysis using 31934-1-AP

    WB analysis using 31934-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31934-1-AP (ZNF787 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 31934-1-AP

    IF Staining of A431 using 31934-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNF787 antibody (31934-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

ZNF787 Polyclonal antibody

ZNF787 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31934-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Zinc finger protein 787, TTF-I-interacting peptide 20, Transcription Termination Factor I Interacting Peptide 20, TIP20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, U-251 cells, RT-4 cells, NIH/3T3 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31934-1-AP targets ZNF787 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ZNF787 fusion protein Ag36052 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 787
别名 Zinc finger protein 787, TTF-I-interacting peptide 20, Transcription Termination Factor I Interacting Peptide 20, TIP20
计算分子量40 kDa
观测分子量40-50 kDa
GenBank蛋白编号BC077728
基因名称 ZNF787
Gene ID (NCBI) 126208
RRIDAB_3670151
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6DD87
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc finger protein 787 (ZNF787, also known as TIP20) is activated in the nucleus and may be involved in transcriptional regulation. It probably is one repressor of neuronal features and can interact with histone deacetylase 1 (HDAC1) in regulating the permeability of the blood-brain barrier (BBB) as well as the pathogenesis of Alzheimer's disease (PMID: 33057419; 38329647).

实验方案

Product Specific Protocols
WB protocol for ZNF787 antibody 31934-1-APDownload protocol
IF protocol for ZNF787 antibody 31934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols