验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
25556-1-AP targets ZNF695 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag22202 Product name: Recombinant human ZNF695 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 88-133 aa of BC023527 Sequence: LSSYLTEDILPEQGLQVSFQKVMLRRYERCCLEKLRLRNDWEIVAM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 695 |
| 别名 | SBZF3 |
| 计算分子量 | 515 aa, 60 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC023527 |
| 基因名称 | ZNF695 |
| Gene ID (NCBI) | 57116 |
| RRID | AB_2880130 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IW36 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNF695 is a KRAB-containing zinc finger transcription factor localized in the nucleus. It binds DNA and mediates transcriptional repression, and its dysregulation participates in tumor progression across multiple malignancies. Elevated ZNF695 promotes cancer cell proliferation, accelerates cell cycle and suppresses apoptosis by activating NEK2 and PI3K/Akt/mTOR pathways. It acts as a prognostic biomarker correlated with tumor immune infiltration in colorectal, cervical, breast and endometrial cancers. Its expression varies among tumor subtypes, associating with aggressive tumor phenotypes (PMID: 38441026; PMID: 40682845).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF695 antibody 25556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncol Rep Zinc finger protein 695 facilitates the proliferation of colorectal cancer cells through activation of the NEK2 and PI3K/Akt/mTOR signaling pathways
| ||
J Cancer Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma. |
