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验证数据展示

  • WB analysis using 25794-1-AP

    WB analysis using 25794-1-AP

    Rat liver tissue lysates were subjected to SDS PAGE followed by western blot with 25794-1-AP (ZNF672 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 25794-1-AP

    IF Staining of HeLa using 25794-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZNF672 antibody (25794-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ZNF672 Polyclonal antibody

ZNF672 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25794-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

zinc finger protein 672

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat liver tissue, mouse liver tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25794-1-AP targets ZNF672 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22886

Product name: Recombinant human ZNF672 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 103-452 aa of BC050348

Sequence: TCGRRFPHLPALLLHRRRQHLPERPRRCPLCARTFRQSALLFHQARAHPLGTTSDPAAPPHRCAQCPRAFRSGAGLRSHPRIHVSRSPTRPRVSDAHQCGVCGKCFGKSSTLTRHLQTHSGEKPFKCPECGKGFLESATLVRHQRTHTGEKPYACGDCGRCFSESSTLLRHRRSHQGERPHACATCGKGFGQRSDLVVHQRIHTGEKPFACPECGRRFSDRSDLTKHRRTHTGEKPYRCELCGKRFTCVSNLNVHRRNHAGHKPHKCPECSKAFSVASKLALHRKTHLGERPAECAECGKCFSHSRSLSQHQRAHTRARTAAAVAIQSAVGTALVFEGPAEQEKPGFSVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 672
别名 zinc finger protein 672
计算分子量 452 aa, 50 kDa
观测分子量50-60 kDa
GenBank蛋白编号BC050348
基因名称 ZNF672
Gene ID (NCBI) 79894
RRIDAB_3742160
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ499Z4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF672 (Zinc Finger Protein 672) belongs to the Krueppel C2H2-type zinc-finger protein family, a large class of transcription factors characterized by tandem C2H2-type zinc finger domains that mediate sequence-specific DNA binding.

实验方案

Product Specific Protocols
IF protocol for ZNF672 antibody 25794-1-APDownload protocol
WB protocol for ZNF672 antibody 25794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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