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验证数据展示

  • WB analysis using 21329-1-AP

    WB analysis using 21329-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21329-1-AP (ZNF671 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 21329-1-AP

    IF Staining of U-251 using 21329-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ZNF671 antibody (21329-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

ZNF671 Polyclonal antibody

ZNF671 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21329-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Zinc finger protein 671

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells, SKOV-3 cells, U-251 cells
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21329-1-AP targets ZNF671 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag15319

Product name: Recombinant human ZNF671 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 207-556 aa of BC025728

Sequence: FDQHQNQPNGGKLFPRKEGRDSVKSCRVHVPEKTLTCGKGRRDFSATSGLLQHQASLSSMKPHKSTKLVSGFLMGQRYHRCGECGKAFTRKDTLARHQRIHTGERPYECNECGKFFSQSYDLFKHQTVHTGERPYECSECGKFFRQISGLIEHRRVHTGERLYQCGKCGKFFSSKSNLIRHQEVHTGARPYVCSECGKEFSRKHTLVLHQRTHTGERPYECSECGKAFSQSSHLNVHWRIHSSDYECSRCGKAFSCISKLIQHQKVHSGEKPYECSKCGKAFTQRPNLIRHWKVHTGERPYVCSECGREFIRKQTLVLHQRVHAGEKLKSVANVGESLRPMPLTYYMVGN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 671
别名 Zinc finger protein 671
计算分子量 556 aa, 63 kDa
观测分子量 61-70 kDa
GenBank蛋白编号BC025728
基因名称 ZNF671
Gene ID (NCBI) 79891
RRIDAB_10755298
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAW3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ZNF671 antibody 21329-1-APDownload protocol
WB protocol for ZNF671 antibody 21329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

Epigenetic mediated zinc finger protein 671 downregulation promotes cell proliferation and tumorigenicity in nasopharyngeal carcinoma by inhibiting cell cycle arrest.

Authors - Jian Zhang
  • KD Validated
View Article
humanWB

Int J Biol Sci

Increased methylation of ZNF671 suppresses tumor progression by promoting MAPK6 transcription in laryngeal carcinoma

Authors - Wei Zhang
View Article
humanWB

Front Oncol

Epigenetic-Mediated Downregulation of Zinc Finger Protein 671 (ZNF671) Predicts Poor Prognosis in Multiple Solid Tumors.

Authors - Jian Zhang
View Article
humanWB

Front Oncol

The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing.

Authors - Jian Zhang
View Article
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