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验证数据展示

  • WB analysis of HL-60 using 25797-1-AP

    WB analysis of HL-60 using 25797-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25797-1-AP (ZNF649 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 25797-1-AP

    WB analysis using 25797-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25797-1-AP (ZNF649 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ZNF649 Polyclonal antibody

ZNF649 Polyclonal Antibody for WB, ELISA
Cat No. 25797-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Zinc finger protein 649

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HL-60 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25797-1-AP targets ZNF649 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22700

Product name: Recombinant human ZNF649 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 66-183 aa of BC005368

Sequence: LWTLEDEIHSPAHPEIEKADDHLQQPLQNQKILKRTGQRYEHGRTLKSYLGLTNQSRRYNRKEPAEFNGDGAFLHDNHEQMPTEIEFPESRKPISTKSQFLKHQQTHNIEKAHECTDC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 649
别名 Zinc finger protein 649
计算分子量 505 aa, 58 kDa
观测分子量 50-57 kDa
GenBank蛋白编号BC005368
基因名称 ZNF649
Gene ID (NCBI) 65251
RRIDAB_2880241
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BS31
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF649, also named as Zinc finger protein 649, is a 505 amino acid protein, which contains The one KRAB domain and belongs to the krueppel C2H2-type zinc-finger protein family. ZNF649 is highly expressed in heart, skeletal muscle, and brain and lower expression in liver, lung, kidney, pancreas and placenta. ZNF649 as a regulator of transcriptional factor complexes may suppress SRE and AP-1 transcription activities by growth factor signaling pathways.

实验方案

Product Specific Protocols
WB protocol for ZNF649 antibody 25797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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