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验证数据展示

  • WB analysis of HeLa using 25817-1-AP

    WB analysis of HeLa using 25817-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25817-1-AP (ZNF639 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25817-1-AP

    IP experiment of HeLa using 25817-1-AP

    IP result of anti-ZNF639 (IP:25817-1-AP, 4ug; Detection:25817-1-AP 1:300) with HeLa cells lysate 3960ug.

ZNF639 Polyclonal antibody

ZNF639 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25817-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

6230400O18Rik, ANC 2H01, ANC_2H01, ZASC1, zinc finger protein 639, Zinc finger protein ANC_2H01, Zinc finger protein ZASC1, ZNF639

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25817-1-AP targets ZNF639 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19854

Product name: Recombinant human ZNF639 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 136-485 aa of BC026181

Sequence: EDVPIAVEVHAISEDYDIETENNSSESLQDQTDEEPPAKLCKILDKSQALNVTAQQKWPLLRANSSGLYKCELCEFNSKYFSDLKQHMILKHKRTDSNVCRVCKESFSTNMLLIEHAKLHEEDPYICKYCDYKTVIFENLSQHIADTHFSDHLYWCEQCDVQFSSSSELYLHFQEHSCDEQYLCQFCEHETNDPEDLHSHVVNEHACKLIELSDKYNNGEHGQYSLLSKITFDKCKNFFVCQVCGFRSRLHTNVNRHVAIEHTKIFPHVCDDCGKGFSSMLEYCKHLNSHLSEGIYLCQYCEYSTGQIEDLKIHLDFKHSADLPHKCSDCLMRFGNERELISHLPVHETT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 639
别名 6230400O18Rik, ANC 2H01, ANC_2H01, ZASC1, zinc finger protein 639, Zinc finger protein ANC_2H01, Zinc finger protein ZASC1, ZNF639
计算分子量 485 aa, 56 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC026181
基因名称 ZNF639
Gene ID (NCBI) 51193
RRIDAB_2880251
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UID6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF639, also named as ZASC1, is a 485 amino acid protein, which locates in the nucleus and contains 8 C2H2-type zinc fingers and belongs to the krueppel C2H2-type zinc-finger protein family. ZNF639 binds DNA and may function as a transcriptional repressor.

实验方案

Product Specific Protocols
IP protocol for ZNF639 antibody 25817-1-APDownload protocol
WB protocol for ZNF639 antibody 25817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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