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验证数据展示

  • WB analysis using 31300-1-AP

    WB analysis using 31300-1-AP

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 31300-1-AP (ZNF638 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 31300-1-AP

    IP experiment of Jurkat using 31300-1-AP

    IP result of anti-ZNF638 (IP:31300-1-AP, 4ug; Detection:31300-1-AP 1:3000) with Jurkat cells lysate 1360 ug.

ZNF638 Polyclonal antibody

ZNF638 Polyclonal Antibody for WB, IP, ELISA
Cat No. 31300-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

CTCL associated antigen se33 1, NP220, Nuclear protein 220, ZFML, zinc finger protein 638

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy ZNF638 Ready-To-Use IHC Kit. ZNF638 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31300-1-AP targets ZNF638 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35296

Product name: Recombinant human ZNF638 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1777-1978 aa of NM_014497.4

Sequence: VDEVGEEEDGDNDLKVELAQSKNDHPTDKKGNRKKRAVDTKKTKLESLSQVGPVNENVMEEDLKTMIERHLTAKTPTKRVRIGKTLPSEKAVVTEPAKGEEAFQMSEVDEESGLKDSEPERKRKKTEDSSSGKSVASDVPEELDFLVPKAGFFCPICSLFYSGEKAMTNHCKSTRHKQNTEKFMAKQRKEKEQNEAEERSSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 638
别名 CTCL associated antigen se33 1, NP220, Nuclear protein 220, ZFML, zinc finger protein 638
计算分子量221kDa
观测分子量 270-300 kDa
GenBank蛋白编号NM_014497.4
基因名称 ZNF638
Gene ID (NCBI) 27332
RRIDAB_3669937
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ14966
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404). The ZNF638 protein is modified and exhibits a molecular weight of ~300 kDa (PMID: 33040080)

实验方案

Product Specific Protocols
IP protocol for ZNF638 antibody 31300-1-APDownload protocol
WB protocol for ZNF638 antibody 31300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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