• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of SH-SY5Y using 25592-1-AP

    WB analysis of SH-SY5Y using 25592-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25592-1-AP (ZNF549 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 25592-1-AP

    IF Staining of A549 using 25592-1-AP

    Immunofluorescent analysis of A549 cells using 25592-1-AP (ZNF549 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ZNF549 Polyclonal antibody

ZNF549 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25592-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inSH-SY5Y cells, A549 cells, HeLa cells, SMMC-7721 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25592-1-AP targets ZNF549 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22371

Product name: Recombinant human ZNF549 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 109-236 aa of BC060863

Sequence: CILVMKDILYLSEHQGTLPWQKPYTSVASGKWFSFGSNLQQHQNQDSGEKHIRKEESSALLLNSCKIPLSDNLFPCKDVEKDFPTILGLLQHQTTHSRQEYAHRSRETFQQRRYKCEQVFNEKVHVTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 549
别名
计算分子量 640 aa, 74 kDa
观测分子量 74 kDa
GenBank蛋白编号BC060863
基因名称 ZNF549
Gene ID (NCBI) 256051
RRIDAB_2880147
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P9A3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF549 (zinc finger protein 549), belonging to the kruppel C2H2-type zinc-finger protein family, is a 640 amino acid nuclear protein that exists as 2 alternatively spliced isoforms and may be involved in transcriptional regulation in cancer disease. Overexpression of ZNF549 inhibit the ability of COAD cell proliferation and migration (PMID: 33028966).

实验方案

Product Specific Protocols
IF protocol for ZNF549 antibody 25592-1-APDownload protocol
WB protocol for ZNF549 antibody 25592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...