验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21365-1-AP targets ZNF543 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag15830 Product name: Recombinant human ZNF543 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 132-225 aa of BC101015 Sequence: EVHLKIGIGPQRGKLLEKMSSERDGLGSDDGVCTKITQKQVSTEGDLYECDSHGPVTDALIREEKNSYKCEECGKVFKKNALLVQHERIHTQVK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 543 |
| 别名 | Zinc finger protein 543 |
| 计算分子量 | 600 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC101015 |
| 基因名称 | ZNF543 |
| Gene ID (NCBI) | 125919 |
| RRID | AB_3742030 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08ER8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNF543 is a member of the Kruppel-associated box (KRAB) containing zinc finger proteins family, which function as transcription factors. ZNF543 has a wide range of physiological functions in a variety of cellular processes, which including apoptosis, cell proliferation and differentiation (PMID: 35467255, PMID: 34975305).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF543 antibody 21365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
