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验证数据展示

  • WB analysis using 21317-1-AP

    WB analysis using 21317-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21317-1-AP (ZNF536 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ZNF536 Polyclonal antibody

ZNF536 Polyclonal Antibody for WB, ELISA
Cat No. 21317-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

KIAA0390

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA172 cells, U-118 MG cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21317-1-AP targets ZNF536 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ZNF536 fusion protein Ag15971 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 536
别名 KIAA0390
计算分子量 1300 aa, 141 kDa
观测分子量130~150 kDa
GenBank蛋白编号BC150171
基因名称 ZNF536
Gene ID (NCBI) 9745
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15090
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF536 (Zinc finger protein 536) is a highly conserved zinc finger protein, acting as DNA-binding transcription suppressor, and negatively regulating neuron differentiation. ZNF536 is most abundant in the brain and, in particular, is expressed in the developing central nervous system and dorsal root ganglia and localized in the cerebral cortex, hippocampus, and hypothalamic area (PMID: 19398580).

实验方案

Product Specific Protocols
WB protocol for ZNF536 antibody 21317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols