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验证数据展示

  • WB analysis using 23716-1-AP (same clone as 23716-1-PBS)

    WB analysis using 23716-1-AP (same clone as 23716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23716-1-AP (ZNF521 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23716-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 23716-1-AP (same clone as 23716-1-PBS)

    IF Staining of A431 using 23716-1-AP (same clone as 23716-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNF521 antibody (23716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23716-1-PBS in a different storage buffer formulation.

ZNF521 Polyclonal antibody, PBS Only

ZNF521 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 23716-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Zfp521, zinc finger protein 521, LYST interacting protein 3, LIP3, Evi3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

23716-1-PBS targets ZNF521 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20540

Product name: Recombinant human ZNF521 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 195-450 aa of BC113648

Sequence: THTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCVYCHELFVEETSLMNHMEQVHSGEKKNSCSICSESFHTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTGPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 521
别名 Zfp521, zinc finger protein 521, LYST interacting protein 3, LIP3, Evi3
计算分子量 1311 aa, 148 kDa
观测分子量190 kDa
GenBank蛋白编号BC113648
基因名称 ZNF521
Gene ID (NCBI) 25925
RRIDAB_3669415
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96K83
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zinc finger protein 521 (EHZF/ZNF521) is a transcription co-factor originally identified for its abundant and selective expression in early progenitors of the human hematopoietic system. In the hematopoietic system ZNF521 is highly expressed in stem and progenitor cells but not in more differentiated precursors or mature leukocytes (PMID: 23907569, 21593590).

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