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验证数据展示

  • IF Staining of HepG2 using 32046-1-AP

    IF Staining of HepG2 using 32046-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF517 antibody (32046-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

ZNF517 Polyclonal antibody

ZNF517 Polyclonal Antibody for IF/ICC, ELISA
Cat No. 32046-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

Zinc finger protein 517

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32046-1-AP targets ZNF517 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZNF517 fusion protein Ag37110 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 517
别名 Zinc finger protein 517
GenBank蛋白编号BC126161
基因名称 ZNF517
Gene ID (NCBI) 340385
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6ZMY9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ZNF family genes are an ancient group of mammalian paralogous homologs, and ZNF517 has been continuously lost during evolution, and although ZNF517 may be involved in transcriptional regulation, its exact function is unknown. A partial deletion and down-regulation of ZNF517 gene expression in cutaneous malignant melanoma (CMM) has been noted.

实验方案

Product Specific Protocols
IF protocol for ZNF517 antibody 32046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols