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ZNF497 Polyclonal antibody

ZNF497 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25178-1-AP

Print datasheet

Synonyms

zinc finger protein 497, ZNF497

验证数据展示

  • WB analysis of mouse testis using 25178-1-AP

    WB analysis of mouse testis using 25178-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25178-1-AP (ZNF497 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25178-1-AP

    IHC staining of human testis using 25178-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 25178-1-AP

    IHC staining of human kidney using 25178-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25178-1-AP is ZNF497 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen ZNF497 fusion protein Ag18114 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 497
Synonyms zinc finger protein 497, ZNF497
Calculated Molecular Weight 498 aa, 55 kDa
Observed Molecular Weight 61-65 kDa
GenBank Accession NumberBC118983
Gene Symbol ZNF497
Gene ID (NCBI) 162968
RRIDAB_2879944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZNH5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF497 antibody 25178-1-APDownload protocol
IHC protocol for ZNF497 antibody 25178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols