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ZNF451 Polyclonal antibody

ZNF451 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 25228-1-AP

Print datasheet

Synonyms

COASTER, KIAA0576, KIAA1702, zinc finger protein 451, ZNF451

验证数据展示

  • IP experiment of HeLa using 25228-1-AP

    IP experiment of HeLa using 25228-1-AP

    IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 1080 ug.

  • WB analysis of HeLa using 25228-1-AP

    WB analysis of HeLa using 25228-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25228-1-AP (ZNF451 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 25228-1-AP

    IHC staining of human placenta using 25228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25228-1-AP (ZNF451 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 25228-1-AP

    IF Staining of HeLa using 25228-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25228-1-AP (ZNF451 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25228-1-AP is ZNF451 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen ZNF451 fusion protein Ag18346 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 451
Synonyms COASTER, KIAA0576, KIAA1702, zinc finger protein 451, ZNF451
Calculated Molecular Weight 1061 aa, 121 kDa
Observed Molecular Weight 121 kDa
GenBank Accession NumberBC021712
Gene Symbol ZNF451
Gene ID (NCBI) 26036
RRIDAB_2879975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4E5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF451 antibody 25228-1-APDownload protocol
IHC protocol for ZNF451 antibody 25228-1-APDownload protocol
IF protocol for ZNF451 antibody 25228-1-APDownload protocol
IP protocol for ZNF451 antibody 25228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Rep

ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression

Authors - Yu Zhang
  • KD Validated
View Article