验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, mouse brain tissue, HeLa cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| ChIP | See 1 publications below |
产品信息
14529-1-AP targets ZNF410 in WB, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6028 Product name: Recombinant human ZNF410 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC050683 Sequence: MLSDELESKPELLVQFVQNTSIPLGQGLVESEAKDITCLSLLPVTEASECSRLMLPDDTTNHSNSSKEVPSSAVLRSLRVNVGPDGEETRAQTVQKSPEFLSTSESSSLLQDLQPSDSTSFILLNLTRAGLGSSAEHLVFVQDEAEDSGNDFLSSESTDSSIPWFLRVQELAHDSLIAATRAQLAKNAKTSSNGENVHLGSGDGQSKDSGPLPQVEKKLKCTVEGCDRTFVWPAHFKYHLKTHRNDRSFICPAEGCGKSFYVLQRLKVHMRTHNGEKPFMCHESGCGKQFTTAGNLKNHRRIHTGEKPFLCEAQGCGRSFAEYSSLRKHLVVHSGEKPHQCQVCGKTFSQS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 410 |
| 别名 | Zinc finger protein APA 1, Zinc finger protein 410, APA1, APA 1, Another partner for ARF 1 |
| 计算分子量 | 52 kDa |
| 观测分子量 | 52-67 kDa |
| GenBank蛋白编号 | BC050683 |
| 基因名称 | ZNF410 |
| Gene ID (NCBI) | 57862 |
| RRID | AB_2257520 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86VK4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNF410 (Zinc Finger Protein 410) is a transcription factor involved in the regulation of gene expression, particularly in the context of fetal hemoglobin (HbF) repression and chromatin remodeling. This protein is highly conserved among mammals and plays a crucial role in erythroid cells. The study found that ZNF410 uniquely silences HbF by activating its sole target gene, CHD4, a known HbF repressor in erythroid cells. (PMID: 40158221)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF410 antibody 14529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression. | ||
EMBO J PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase. | ||
Mol Ther Development of a double shmiR lentivirus effectively targeting both BCL11A and ZNF410 for enhanced induction of fetal hemoglobin to treat β-hemoglobinopathies. | ||
Biomedicines Heteronemin Suppresses Lymphangiogenesis through ARF-1 and MMP-9/VE-Cadherin/Vimentin. | ||
Free Radic Biol Med Critical role of caveolin-1 in intestinal ischemia reperfusion by inhibiting protein kinase C βII |