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验证数据展示

  • IHC staining of mouse liver using 20799-1-AP

    IHC staining of mouse liver using 20799-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20799-1-AP (ZNF397 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZNF397 antibody included
  • IHC staining of mouse liver using 20799-1-AP

    IHC staining of mouse liver using 20799-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20799-1-AP (ZNF397 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZNF397 antibody included
  • WB analysis of PC-3 using 20799-1-AP

    WB analysis of PC-3 using 20799-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20799-1-AP (ZNF397 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ZNF397 Polyclonal antibody

ZNF397 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20799-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, Rat

应用

WB, IHC, ELISA

别名

zinc finger protein 397, Zinc finger protein 47, ZNF397, ZNF47, ZSCAN15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, mouse testis tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ZNF397 Ready-To-Use IHC Kit. ZNF397 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20799-1-AP targets ZNF397 in WB, IHC, ELISA applications and shows reactivity with human, mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, Rat
免疫原

CatNo: Ag14712

Product name: Recombinant human ZNF397 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-275 aa of BC006172

Sequence: MAVESGVISTLIPQDPPEQELILVKVEDNFSWDEKFKQNGSTQSCQELFRQQFRKFCYQETPGPREALSRLQELCYQWLMPELHTKEQILELLVLEQFLSILPEELQIWVQQHNPESGEEAVTLLEDLEREFDDPGQQVPASPQGPAVPWKDLTCLRASQESTDIHLQPLKTQLKSWKPCLSPKSDCENSETATKEGISEEKSQGLPQEPSFRGIKLSRPPKASSAIRWECVSPGSFPGDIIAAEATHSTISCFAINTLPATILPSKNVNRKYFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 397
别名 zinc finger protein 397, Zinc finger protein 47, ZNF397, ZNF47, ZSCAN15
计算分子量 534 aa, 61 kDa
观测分子量 60-63 kDa
GenBank蛋白编号BC006172
基因名称 ZNF397
Gene ID (NCBI) 84307
RRIDAB_2878741
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NF99
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF397 (Zinc Finger Protein 397) is conserved in placental mammals. ZNF397 as a bona fide coactivator of the androgen receptor (AR), essential for the transcriptional program governing AR-driven luminal lineage.

实验方案

Product Specific Protocols
WB protocol for ZNF397 antibody 20799-1-APDownload protocol
IHC protocol for ZNF397 antibody 20799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols