验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, fetal human brain tissue, HepG2 cells, mouse spleen tissue |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25785-1-AP targets ZNF394 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag22655 Product name: Recombinant human ZNF394 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-375 aa of BC051847 Sequence: RALQRALDGTSSQGMVTFEDTAVSLTWEEWERLDPARRDFCRESAQKDSGSTVPPSLESRVENKELIPMQQILEEAEPQGQLQEAFQGKRPLFSKCGSTHEDRVEKQSGDPLPLKLENSPEAEGLNSISDVNKNGSIEGEDSKNNELQNSARCSNLVLCQHIPKAERPTDSEEHGNKCKQSFHMVTWHVLKPHKSDSGDSFHHSSLFETQRQLHEERPYKCGNCGKSFKQRSDLFR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 394 |
| 别名 | ZKSCAN14 |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC051847 |
| 基因名称 | ZNF394 |
| Gene ID (NCBI) | 84124 |
| RRID | AB_2880239 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53GI3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNF394 (zinc finger protein 394) is a C2H2-type zinc finger protein belonging to the Krüppel C2H2-type zinc finger protein family. The protein contains three distinct domains: the N-terminus has two domains, LER and KRAB, and the C-terminus has seven C2H2 zinc finger domains. ZNF394 is predicted to have DNA-binding transcription factor activity and is involved in the transcriptional regulation of RNA polymerase II. Additionally, ZNF394, together with ZIM3, plays a key role in human embryonic development at the 8-cell stage by inhibiting the expression of genes specific to the 4-cell stage, thereby promoting embryonic development.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF394 antibody 25785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
