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ZNF326 Polyclonal antibody

ZNF326 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25147-1-AP

Print datasheet

Synonyms

ZAN75, Zfp326, zinc finger protein 326, ZNF326

验证数据展示

  • WB analysis of Jurkat using 25147-1-AP

    WB analysis of Jurkat using 25147-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 25147-1-AP

    IHC staining of mouse liver using 25147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25147-1-AP (ZNF326 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of mouse liver using 25147-1-AP

    IHC staining of mouse liver using 25147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25147-1-AP (ZNF326 Antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 25147-1-AP

    IF Staining of HeLa using 25147-1-AP

    Immunofluorescent analysis of HeLa cells using 25147-1-AP (ZNF326 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25147-1-AP is ZNF326 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ZNF326 fusion protein Ag18433 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 326
Synonyms ZAN75, Zfp326, zinc finger protein 326, ZNF326
Calculated Molecular Weight 582 aa, 66 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC090866
Gene Symbol ZNF326
Gene ID (NCBI) 284695
RRIDAB_2879923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5BKZ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF326 antibody 25147-1-APDownload protocol
IHC protocol for ZNF326 antibody 25147-1-APDownload protocol
IF protocol for ZNF326 antibody 25147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Sci Rep

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Authors - S Prpar Mihevc
View Article
mouseWB

Toxicol Sci

LncRNA NR_030777 Alleviates Paraquat-induced Neurotoxicity by Regulating Zfp326 and Cpne5.

Authors - Hongyu Yang
View Article