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验证数据展示

  • WB analysis of HL-60 using 23243-1-AP

    WB analysis of HL-60 using 23243-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 23243-1-AP (ZNF311 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23243-1-AP

    IHC staining of human testis using 23243-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23243-1-AP (ZNF311 Antibody) at dilution of 1:50.

  • IHC staining of human kidney using 23243-1-AP

    IHC staining of human kidney using 23243-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 23243-1-AP (ZNF311 Antibody) at dilution of 1:50.

ZNF311 Polyclonal antibody

ZNF311 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23243-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

zf31, ZFP31, zinc finger protein 311, Zinc finger protein zfp 31, ZNF311

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23243-1-AP targets ZNF311 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19624

Product name: Recombinant human ZNF311 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC136745

Sequence: MQEVVLLDESSGPPSQLLWTRQDTQLPQESALLPAPYPAFTKDGSQGNLPQADITLMSQAQESVTFEDVAVNFTNREWQCLTYAQRHLYKDVMLENYGNMVSLGFPFPKPPLISHLEREVDPCVQDPQDRESLSCSYPVSADKMWPENEKASSQQEIFENGEAYWMKFNSLLKVDSRDPKVREVCVQDVKLENQWETSIREKLREEKEGSEEVTCKKGKNQKVLSKNLNPNSKHSQCNKVLIAQKLHECARCGKNFSWHSDLILHEQIHSGEKPHVCNECGKAFKTRNQLSMHRIIHTGEKPFNCTQCGKAFNSRSALCRHKKTHSGEKPHECRDCGKAFKTRNRLCMHQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 311
别名 zf31, ZFP31, zinc finger protein 311, Zinc finger protein zfp 31, ZNF311
计算分子量 666 aa, 76 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC136745
基因名称 ZNF311
Gene ID (NCBI) 282890
RRIDAB_11232217
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JNZ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc finger protein 311 (ZNF311), also named Zinc finger protein zfp-31 (ZFP31), is involved in regulation of transcription. It belongs to the krueppel C2H2-type zinfinger protein family, and contains 14 C2H2-type zinc fingers and 1 KRAB domain.This antibody specifically reacts with ZNF311 protein.

实验方案

Product Specific Protocols
WB protocol for ZNF311 antibody 23243-1-APDownload protocol
IHC protocol for ZNF311 antibody 23243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols