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验证数据展示

  • WB analysis using 24088-1-AP

    WB analysis using 24088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24088-1-AP (ZNF281 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 24088-1-AP

    IF Staining of U2OS using 24088-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZNF281 antibody (24088-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

ZNF281 Polyclonal antibody

ZNF281 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24088-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

GZP1, Transcription factor ZBP 99, ZBP 99, ZBP99, zinc finger protein 281, ZNF281, ZNP 99

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNCCIT cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24088-1-AP targets ZNF281 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21355

Product name: Recombinant human ZNF281 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 695-895 aa of BC060820

Sequence: LSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 281
别名 GZP1, Transcription factor ZBP 99, ZBP 99, ZBP99, zinc finger protein 281, ZNF281, ZNP 99
计算分子量 895 aa, 97 kDa
观测分子量110 kDa
GenBank蛋白编号BC060820
基因名称 ZNF281
Gene ID (NCBI) 23528
RRIDAB_3085722
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9Y2X9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ZNF281 antibody 24088-1-APDownload protocol
WB protocol for ZNF281 antibody 24088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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