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验证数据展示

  • WB analysis using 24088-1-AP

    WB analysis using 24088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24088-1-AP (ZNF281 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 24088-1-AP

    IF Staining of U2OS using 24088-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZNF281 antibody (24088-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

ZNF281 Polyclonal antibody

ZNF281 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24088-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

GC-box-binding zinc finger protein 1, GZP1, Transcription factor ZBP-99, ZBP 99, ZBP99

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNCCIT cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24088-1-AP targets ZNF281 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21355

Product name: Recombinant human ZNF281 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 695-895 aa of BC060820

Sequence: LSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 281
别名 GC-box-binding zinc finger protein 1, GZP1, Transcription factor ZBP-99, ZBP 99, ZBP99
计算分子量 895 aa, 97 kDa
观测分子量110 kDa
GenBank蛋白编号BC060820
基因名称 ZNF281
Gene ID (NCBI) 23528
RRIDAB_3085722
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9Y2X9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF281 (zinc finger protein 281) is a Krüppel family transcription factor containing four C2H2-type zinc finger domains, located in the cell nucleus. It promotes epithelial-mesenchymal transition (EMT) and tumor metastasis by recruiting co-repressors such as CTBP or synergistically with ZEB1 to inhibit the expression of epithelial markers such as E-cadherin. Furthermore, this protein is also involved in maintaining embryonic stem cell pluripotency and regulating neural differentiation.

实验方案

Product Specific Protocols
IF protocol for ZNF281 antibody 24088-1-APDownload protocol
WB protocol for ZNF281 antibody 24088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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