验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
32519-1-AP targets ZNF280B in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | ZNF280B fusion protein Ag38544 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | zinc finger protein 280B |
别名 | Zinc finger protein 632, Zinc finger protein 280B, Zinc finger protein 279, Suppressor of hairy wing homolog 2, SUHW2 |
计算分子量 | 62kDa,543aa |
观测分子量 | 60 kDa |
GenBank蛋白编号 | NM_080764.3 |
基因名称 | ZNF280B |
Gene ID (NCBI) | 140883 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | Q86YH2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNF280B belongs to the family of zinc finger proteins, a transcription factor that is mainly involved in the regulation of gene expression. In prostate cancer cells, ZNF280B induces p53 nuclear export, leading to subsequent proteasomal degradation. Therefore, ZNF280B has pro-growth and pro-survival functions in prostate cancer cells. In addition to this, ZNF280B has a promotional role in gastric and lung cancer development, and its potential as a tumor therapeutic target can be explored in the future.
实验方案
Product Specific Protocols | |
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WB protocol for ZNF280B antibody 32519-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |