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验证数据展示

  • WB analysis using 32519-1-AP

    WB analysis using 32519-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32519-1-AP (ZNF280B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

ZNF280B Polyclonal antibody

ZNF280B Polyclonal Antibody for WB, ELISA
Cat No. 32519-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Zinc finger protein 632, Zinc finger protein 280B, Zinc finger protein 279, Suppressor of hairy wing homolog 2, SUHW2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32519-1-AP targets ZNF280B in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ZNF280B fusion protein Ag38544 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 280B
别名 Zinc finger protein 632, Zinc finger protein 280B, Zinc finger protein 279, Suppressor of hairy wing homolog 2, SUHW2
计算分子量62kDa，543aa
观测分子量60 kDa
GenBank蛋白编号NM_080764.3
基因名称 ZNF280B
Gene ID (NCBI) 140883
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86YH2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF280B belongs to the family of zinc finger proteins, a transcription factor that is mainly involved in the regulation of gene expression. In prostate cancer cells, ZNF280B induces p53 nuclear export, leading to subsequent proteasomal degradation. Therefore, ZNF280B has pro-growth and pro-survival functions in prostate cancer cells. In addition to this, ZNF280B has a promotional role in gastric and lung cancer development, and its potential as a tumor therapeutic target can be explored in the future.

实验方案

Product Specific Protocols
WB protocol for ZNF280B antibody 32519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols