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验证数据展示

  • WB analysis using 31903-1-AP

    WB analysis using 31903-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31903-1-AP (ZNF280A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31903-1-AP

    WB analysis using 31903-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31903-1-AP (ZNF280A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ZNF280A Polyclonal antibody

ZNF280A Polyclonal Antibody for WB, ELISA
Cat No. 31903-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Zinc finger protein 636, Zinc finger protein 280A, Zinc Finger Protein 280, Suppressor of hairy wing homolog 1, SUHW1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, mouse adrenal gland tissue, mouse kidney tissue, rat kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31903-1-AP targets ZNF280A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35942

Product name: Recombinant human ZNF280A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 461-542 aa of BC053901

Sequence: LTLKEEIEHKTKDHQTFKKPEQLQGLPSETKVIIQTSVQPGSSGMASVIVSNTDPQSSPVKTKKKTAMNTRDSRLPCSKDSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 280A
别名 Zinc finger protein 636, Zinc finger protein 280A, Zinc Finger Protein 280, Suppressor of hairy wing homolog 1, SUHW1
计算分子量60 kDa
观测分子量50 kDa
GenBank蛋白编号BC053901
基因名称 ZNF280A
Gene ID (NCBI) 129025
RRIDAB_3670138
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP59817
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc finger proteins are the largest transcription factor family in the human genome and play a significant role in regulating and managing gene expression, including development, differentiation, metabolism, and autophagy (PMID: 18253864; 27411336). Zinc finger protein 280A (ZNF280A, also known as SUHW1, ZNF280, and ZNF636) is a member of the zinc-finger protein family, carrying two consecutive Cys2His2 zinc finger domains (PMID: 33414445). Cys2-His2 (C2H2) zinc finger domains (ZFs) were originally identified as DNA-binding domains, and uncharacterized domains are typically assumed to function in DNA binding (PMID: 18253864).

实验方案

Product Specific Protocols
WB protocol for ZNF280A antibody 31903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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