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验证数据展示

  • WB analysis of HeLa using 11374-1-AP

    WB analysis of HeLa using 11374-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11374-1-AP (ZNF266 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11374-1-AP

    IHC staining of human prostate cancer using 11374-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11374-1-AP (ZNF266 antibody) at dilution of 1:100 (under 10x lens).

ZNF266 Polyclonal antibody

ZNF266 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11374-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HZF1, KIAA2007, zinc finger protein 266, Zinc finger protein HZF1, ZNF266

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11374-1-AP targets ZNF266 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ZNF266 fusion protein Ag1933 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 266
别名 HZF1, KIAA2007, zinc finger protein 266, Zinc finger protein HZF1, ZNF266
计算分子量 670 aa, 70 kDa
观测分子量 50~55+70~75 kDa
GenBank蛋白编号BC017407
基因名称 ZNF266
Gene ID (NCBI) 10781
RRIDAB_2257588
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14584
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF266, also named as HZF1, was expressed in ubiquitous tissues and various hematopoietic cell lines. Expression of ZNF266 in K562 cells was increased after being induced by hemin or phorbol myristate acetate (PMA), suggesting that HZF1 play important roles in erythroid and megakaryocytic differentiation.

实验方案

Product Specific Protocols
WB protocol for ZNF266 antibody 11374-1-APDownload protocol
IHC protocol for ZNF266 antibody 11374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols